LEARNING OUTCOMES
1. Comprehension of fundamental settings of radiobiology in the interpretation of specific radiobiology concepts as well as developing predisposition for further learning
2. Comprehension of mechanism of isotopes with regard to their application
3. Using the basic concepts of radioactivity and their understanding in the field of biology and radiation protection
4. Understanding the role and application of various sources of radiation in medicine and industry
5. Utilization of the basic facts about the importance of measuring the level of ionizing radiation in the environment and population
6. Comprehension and evaluation of the interaction energy radiation with biological media
7. Interpretation and utilization of important facts about the sensitivity of cells exposed to radiation
8. The utilization of dose response curves in analyzing sensitivity of cells, tissue and organs exposed to radiation
9. Applying the basic settings of radio sensitivity in the interpretation and application of ionizing radiation modifiers
10. Comprehension of epidemiological studies and analysis of a wide range of facts potentially exposed to radiation
COURSE CONTENT
LECTURES
1. Introductory into radiobiology: what it represents, with the emphasis put on harmful biological effects, review of major scientific achievements, radiation types.
2. Isotopes: stable and unstable, application of radioisotopes in medical diagnostics and therapy.
3. Radioactivity: measuring units, absorbed dose, dose rate, exposure, quality assurance, linear energy transfer (LET).
4. Environmental radiation sources: natural and man-made sources, application of radiation sources in medical and diagnostics and therapy, radiation (external and internal).
5. Measurements of ionizing radiation: measuring devices (dosimeters, counters, track visualization devices, personal dosimetry).
6.7. Physical properties of ionizing radiation: direct and indirect interaction of radiation energies with biological media, interaction with biological macromolecules.
8.9. Radio sensitivity of cells: effects on intracellular structures and organelles, changes in DNA molecule.
10. Survival curves: viruses, bacterial, mammalian cells, Target theory.
11. Radio sensitivity of chromosomes: mechanisms of DNA-damage, changes of chromosomal structure, biological consequences.
12. Tissue radio sensitivity: delays effects of radiation (type of acute radiation syndromes).
13. Modificators of radio sensitivity: chemical radio protectors, radiosensibilizators, mechanism underlying radio sensibility, detection and measurements.
14. Bio monitoring: exposure of general and occupationally exposed population of the Republic of Croatia.
15. Radiofrequency-microwave radiation.
PRACTICAL TRAINING
Introduction
Introduction to instruments for detection and measurement of ionizing radiation (GM detector, scintillation device, thermoluminescent dosimeters).
In different experimental models: in vivo and ex vivo determination of the biological effects of ionizing radiation. The analysis of cytological slides: Analysis of chromosomal aberrations, micronucleus test analysis, micro gel electrophoresis of single cells, Cytotoxicity assay
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- Down SB, Tilson ER. Practical Radiation Protection and Applied Radiobiology, 2nd ed. Saunder WB Company, 1999, Toronto
Polk C, Postow E. Biological Effects of Electromagentic Fields,2 nd ed. CRC Press, 1996, Washington, USA.
Nataraajn AT. 2002. Chromosome aberrations:past,present and future. Mut res 504:3-16-
Fenech M. 2006. Cytokinesis-block micronucleus assay evolves into a cytome assay of chromosmal instability, mitotic dysfunction and cell death. Mut res 600 58-66
Collins A et al. 2008. The comet assay:topical issues. Mutagenesis 23;143-151.
- IAEA; Biological Radiation Effects,Springer Verlag.1990.
Slater RJ. 1990. Radioisotopes in Biology,IRL Press Oxford
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