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Bioanalytics

Code: 128621
ECTS: 5.0
Lecturers in charge: doc. dr. sc. Mario Cindrić - Lectures
Lecturers: Amela Hozić - Seminar
Take exam: Studomat
Load:

1. komponenta

Lecture typeTotal
Lectures 30
Seminar 15
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
Course will cover nine subjects, each teaching unit will take 3 h. All students must complete additional activities related to regular weekly assignment (2h per student). After each finished unit students in group of three will cover regular weekly assignment connected with the next teaching unit. Microseparation techniques and sample preparation (capillary and nano-techniques applied to sample preparation, purification and concentration, derivatization and sensitive separation). Separation techniques used for quantitative and qualitative analysis and isolation (Capillary electrophoresis, 2-D gel electrophoresis, liquid, ion and immunoaffinity chromatography, Ultracentrifugation and Molecular Characterization and Field-Flow Fractionation). Chemical and enzymatic hydrolysis and proteolysis: Top-down and Bottom-up approach; Methods for protein sequencing (Edman degradation, amino acid analysis, enzymatic cleavages, sequencing in gas-phase, prosthetic groups determination). Posttranslational modifications (Phosphorylation, Glycosylation, Acetylation, Ubiquitination, Disulfide bridges, Sulphatation, Oxidation, Deamidation etc., micro?and macro-heterogeneity and detection methods). Quantitative PCR (qPCR, multiplex qPCR, real-time PCR, reverse transcription quantitative PCR, RT-qPCR). Immunochemical detection methods (Western blot, ELISA, usage of polyclonal and monoclonal antibodies). High-throughput analysis (purpose of HTA, HTA profiling, robotics automation, differential display technique). Bioinformatics and mass spectrometry (peptide and protein databases, search engines and mass spectrometry, MASCOT, ProteinProspector, OMSSA etc)

LEARNING OUTCOMES:
Students will be able to:
- demonstrate understanding of basic principle of bioanalysis technology assignment, concept, vocabulary and terminology related to macromolecules bioanalysis
- classify biomolecules in relation to separation techniques applications
- list and explain numerous instrumental techniques essential for successful qualitative and quantitative analysis
- prepare suitable samples relative to biomolecule typization and explain biochemical processes and chemical mechanisms related to desired bioanalysis outcome
- resolve complex biopolymer mixture analysis problems in theory and practice
Literature:
  1. 1. Microcharacterization of Proteins, Roland Kellner, Friedrich Lottspeich, Helmut E. Meyer, WILLEY VCH, 2nd Edition, 1999.
    2. Medicina, Vol.45 No.3, 2009 (http://hrcak.srce.hr/medicina).
    3. Principles and practice of bioanalysis, Richard F Venn, 2nd Edition, 2008.
2. semester
Izborni predmeti izvan odabranih grana - Mandatory smjer - Analytical and inorganic chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Analytical and organic chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Analytical and physical chemistry
Izborni predmeti biokemija - Mandatory smjer - Analytical chemistry and biochemistry
Izborni predmeti biokemija - Mandatory smjer - Biochemistry and organic chemistry
Izborni predmeti biokemija - Mandatory smjer - Biochemistry and physical chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Inorganic and physical chemistry
Izborni predmeti biokemija - Mandatory smjer - Inorganic chemistry and biochemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Physical and organic chemistry

4. semester
Izborni predmeti izvan odabranih grana - Mandatory smjer - Analytical and inorganic chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Analytical and organic chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Analytical and physical chemistry
Izborni predmeti biokemija - Mandatory smjer - Analytical chemistry and biochemistry
Izborni predmeti biokemija - Mandatory smjer - Biochemistry and organic chemistry
Izborni predmeti biokemija - Mandatory smjer - Biochemistry and physical chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Inorganic and physical chemistry
Izborni predmeti biokemija - Mandatory smjer - Inorganic chemistry and biochemistry
Izborni predmeti izvan odabranih grana - Mandatory smjer - Physical and organic chemistry
Consultations schedule: