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Enzymes: Kinetics and Reaction Mechanisms

Code: 152831
ECTS: 8.0
Lecturers in charge: prof. dr. sc. Ita Gruić Sovulj - Lectures
dr. sc. Zrinka Kovarik - Lectures
dr. sc. Zoran Radić - Lectures
Take exam: Studomat
Load:

1. komponenta

Lecture typeTotal
Lectures 20
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
Chemical catalysis (transition state theory, principles of catalysis, covalent catalysis, kinetic isotope effect); Enzyme catalysis; The basic equations of enzyme kinetics (steady-state kinetics, Michaelis-Menten model, inhibition, multisubstrate systems); Measurements and magnitude of individual rate constants (conventional methods, rapid methods-stopped flow, relaxation, quenching, analysis of pre-steady state and relaxation kinetics); The pH dependence of enzyme catalysis; Practical methods for kinetics and equilibria; Detection of intermediates in enzymatic reactions; Irreversible inhibition; Allosteric interactions (positive and negative cooperativity, Hill equation); Stereochemistry of enzymatic reactions; Enzyme-substrate complementarity and the use of binding energy in catalysis; Specificity of enzyme catalysis (limits on specificity; editing or proofreading mechanisms); Catalytic RNA; Selected examples of enzymatic reactions.
Literature:
  1. LITERATURE:
    D. L. Nelson, M. M. Cox, Lehninger principles of biochemistry, W. H. Freeman & Co., 7th ed. New York, 2017.
    Fersht, Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding, W. E. Freeman and Company, New York, 1999.
    Cornish-Bowden, Fundamentals of Enzyme Kinetics, Portland Press, London, 2004.
    H. Segel, Enzyme Kinetics : Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems, Wiley Classics Library Edition, 1993
  2. ADDITIONAL LITERATURE:
    D.L. Purich, Enzyme kinetics: Catalysis and Control, Elsevier Inc, 2010

    revijalni članci i znanstvene monografije
1. semester
Izborni kolegiji za smjer analitička kemija - Mandatory smjer - Analytical chemistry
Temeljni kolegiji iz biokemije - Mandatory smjer - Biochemistry
Izborni kolegiji za smjer anorganska i strukturna kemija - Mandatory smjer - Inorganic and structural chemistry
Izborni kolegiji za smjer organska kemija - Mandatory smjer - Organic chemistry
Izborni kolegiji za smjer fizikalna kemija - Mandatory smjer - Physical chemistry

3. semester
Izborni predmeti doktorski studij kemije - Mandatory smjer - Analytical chemistry
Izborni predmeti doktorski studij kemije - Mandatory smjer - Biochemistry
Izborni predmeti doktorski studij kemije - Mandatory smjer - Inorganic and structural chemistry
Izborni predmeti doktorski studij kemije - Mandatory smjer - Organic chemistry
Izborni predmeti doktorski studij kemije - Mandatory smjer - Physical chemistry
Consultations schedule: