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Instrumental analysis 2

Code: 43975
ECTS: 5.0
Lecturers in charge: prof. dr. sc. Nives Galić
Lecturers: prof. dr. sc. Nives Galić - Seminar
Take exam: Studomat
Load:

1. komponenta

Lecture typeTotal
Lectures 30
Seminar 15
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
COURSE CONTENT:
Mass spectrometry: Introduction, Ion Sources, Mass Analyzers, Analysis of Small Molecules, Analysis of macromolecules.
Chromatography: Introduction to Chromatographic Separations, Gas Chromatography, Liquid Chromatography, Supercritical Fluid Chromatography, Chromatographic columns, Detectors, Hyphenated Techniques (GC-MS, LC-MS), applications.
Immunoassay: Introduction, Assay formats, Immunoprecipitation, Aglutination, Aglutination Inhibition, Labels (Radioactive, Fluorescent and Enzyme labels).
Enzymes in Analytical Chemistry: Characteristics as analytical reagens, Determination by Stoichiometric Methods, Determination by Kinetic Methods.
Chemical Sensors: Introduction, Potentiometric Sensors, Optodes, Biosensors.
EXPECTED COMPETENCES TO BE ACQUIRED:
1. To explain the principles of mass spectrometry.
2. To compare ion sources and mass analyzers as well.
3. To interpret mass spectra.
4. To explain gas, liquid and supercritical fluid chromatography.
5. To interpret chromatogram.
6. To explain immunoassay formats for qualitative and quantitative analyses.
7. To choose an adequate immunoassay according to analyte concentration.
8. To explain the principles of chemical sensors.
9. To explain potentiometric methods for determination of ions in complex samples.
10. To choose adequate instrumental technique and method for qualitative and/or quantitative analyses of different samples.
Literature:
  1. 1. D. A. Skoog, F. J. Holler, T. A. Nieman: Principles of Instrumental Analysis, Harcourt College Publishers, Philadelphia,
    1998.
    2. N. Galić: Imunoanalitičke metode, skripta, Zavod za analitičku kemiju, Kemijski odsjek, PMF, Zagreb, 2003.
    3. N. Galić: Spektrometrija masa, skripta, Zavod za analitičku kemiju, Kemijski odsjek, PMF, Zagreb, 2004.
    4. N. Galić, V. Drevenkar: Kromatografija, skripta, Zavod za analitičku kemiju, Kemijski odsjek, PMF, Zagreb, 2005.
  2. N. Galić: Spektrometrija masa, interna skripta, KO PMF, Zagreb, 2004.
  3. N. Galić, V. Drevenkar: Kromatografija, skripta, KO PMF, Zagreb, 2005.
  4. R. Kellner, J. M. Mermet, M. Otto, H. M. Widmer: Analytical Chemistry, Wiley, Weinheim, 1998.
  5. D. A. Skoog, F. J. Holler, T. A. Nieman: Principles of Instrumental Analysis, Harcourt College Publishers, Philadelphia, 1998.
2. semester
Mandatory course - Regular study - Analytical and inorganic chemistry
Mandatory course - Regular study - Analytical and organic chemistry
Mandatory course - Regular study - Analytical and physical chemistry
Mandatory course - Regular study - Analytical chemistry and biochemistry
Izborni predmeti izvan odabranih grana - Regular study - Biochemistry and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Biochemistry and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic chemistry and biochemistry
Izborni predmeti izvan odabranih grana - Regular study - Physical and organic chemistry

4. semester
Izborni predmeti izvan odabranih grana - Regular study - Biochemistry and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Biochemistry and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic chemistry and biochemistry
Izborni predmeti izvan odabranih grana - Regular study - Physical and organic chemistry
Consultations schedule: