Dynamics and structure of biological membranes: General principles, composition, dynamics, structure and organisation of biological membranes. Structural composition of membrane lipids and proteins. Integral and peripheral membrane proteins, interactions with the membrane lipids. Asymmetrical lipid composition in the outer and inner part of membrane bilayer: synthesis, organisation and transport of membrane lipids. Carbohydrates of the cell membrane: lectins, selectins, modification of carbohydrates in the Golgi apparatus, glycosylation of lysosomal enzymes, diseases related to impaired glycosylation, erythrocyte cell membrane, spectrin, cytoskeleton, anion channel-protein band 3, ABO antigens, structure of A, B, and H antigens. Transport of small molecules across cell membrane: driving forces and gradients, protein carriers, ion channels, gap junctions, control of opening and closing, acetylcholine receptor, structure and function of voltage gated Na+ channel, K+ channel, specificity and selactivity of ion transporters, carrier transport, symport, antiport, transport ATPases, MDR-multidrug resistance transporters of drugs. Families of transport proteins, ABC transporters, CFTR, peptide transporters, Ca2+ pumps, glucose transporters, kinetics of Na+/glucose co-transport, asymmetrical distribution of protein transporters. Specific proteins of the intracellular organelles: mechanism of the intracellular membrane synthesis, cytosol derived proteins, protein and peptides specific for nucleus, mitochondria, peroxisomes, and endoplasmic reticulum. Transport of water across the cell membrane, osmotic pressure, epithelial renal cells, aquaporin. Bacterial cell walls-structure and biological activity: Gram positive and Gram negative bacteria. Endotoxins-structure and biological activity. Peptidoglycans-structure, enzymatic degradation, biological characteristics and influence on immunological system. Artificial membranes: liposomes as a model system of lipid bilayer. Preparation, characterisation and application in biology and medicine.
description of instruction methods lectures or tutorials, depending on number os students
description of course/module requirements oral examination and presentation of seminar on the selected topics
- OBVEZNA LITERATURA:
D. Voet, J. G. Voet, BIOCHEMISTRY 4th ed., J. Wiley & Sons, New York 2011.
J. M. Berg, J. L. Tymoczko, L. Stryer, BIOCHEMISTRY 7th ed., W. H. Freeman & Co., New York 2012.
B.Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, P. Walter, MOLECULAR BIOLOGY OF THE CELL 4th ed., Garland Publishing, Inc., New York 2002.
Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, J.D. Watson: Molecular Biology of the Cell. 5th. izd, Garland Publ., New York 2008.
Ferramosca A, Zara V: Biogenesis of mitochondrial carrier proteins: molecular mechanisms of import into mitochondria. Biochim Biophys Acta. 2013 Mar;1833(3):494-502. Review.
- DOPUNSKA LITERATURA:
revijalni članci i znanstvene monografije