Experimental Design of High Throughput Experiments


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Experimental Design of High Throughput Experiments

Code: 227707
ECTS: 5.0
Lecturers in charge: prof. dr. sc. Kristian Vlahoviček
izv. prof. dr. sc. Rosa Karlić
Lecturers: Doris Brkić , mag. biol. mol. - Practicum
Paula Štancl , mag. biol. mol. - Practicum
Take exam: Studomat

1. komponenta

Lecture typeTotal
Lectures 30
Practicum 30
* Load is given in academic hour (1 academic hour = 45 minutes)
Course objective:
Explain the advantages and disadvantages of using high-throughput sequencing technologies with an emphasis on recommendations when designing experiments, with the aim of obtaining quality data for downstream analysis
Overview of the most commonly used high-throughput sequencing technologies (whole-genome DNA sequencing, RNA sequencing, genome methylation analysis, characterization of DNA-protein interactions, metagenomics and metatranscriptomics, single-cell mRNA and DNA sequencing)
- Recommendations for designing genome-wide DNA sequencing experiments and targeted resequencing of parts of DNA
- RNA sequencing: recommendations in the design of transcriptome, microRNA, long non-coding RNA and ribosome profiling experiments
- Recommendations when designing an experiment in which methylation profiles are determined, design of a ChIP-Seq experiment
- Design of metagenomic and metatranscriptomic experiments
- Design of single-cell mRNA and DNA experiments
- Designing HiC experiments
2. semester
Izborni predmeti - Regular study - Molecular Biology
Consultations schedule: