Learning outcomes:
1 To put in relation knowledge of Plant developmental biology, Cell biology and genetics
2 Development of holistic picture of the coordination of plant development with understanding and sistematization of mechanisms involved.
3 Clarification of cell communication, intrinsic and extrinsic informations, developmental role of cell wall, importance of cell and cell position (longitudinal and radial) in development, enviromental signals, growth regulators and their mechanisms of action
4 Establishment of polarity and identity of the cell.
Lectures:
1 Plant life cycle(2 hour) alternation of generation; gametophyte development; sporophyte development
2 Polarity of cell and plant body (2 hour) cell wall, plasmodesmata, asymmetric cell division
3 Intrinsic informations (2 hours) Lineage; Relation between age and position
4 Extrinsic informations (4 hours) Establishment of polarity in embryogenesis: Longitudinal axis of Fucus embryo; Longitudinal axis of Arabidopsis embryo; Radial axis of arabidopsis embryo; 5 Axis development in the leaf and flower
6 Positional informations (4 hours) Position relative to a particular cell, tissue and Organ ((trichomes, root hairs, phylotaxy)
7Enviromental factors (4 hours) Light developmental responses to light
8 Growth regulators (6 hours) Auxins, giberelins, Cytokinins, Apscisic acid, ethylene, Brasinosteroides
Practical courses:
1 Plant transformation and applications Biolistic transformation (preparation of gold particles, bombardment andmonitoring)
2 Gene expression analysis in different vegetative and germinative tissues: RNA isolation; direct MRNA isolation from single reproductive cell, RTPCR, gene-specific PCR; Subcellular protein Seminars (each seminar 1 hour):
Initiation and maintance of the shoot apical meristem
Transition from embryogenic to postembryogenic development
Phase transition in post embryogenic development
Phylotaxy and golden ration
Shoot branching
Laser ablation of cells in Arabidopsis root tip
Green whitegreen periclinal chimeras
Mutation affecting division pattern
Mutation affecting the rate of leaf initiation
Control of cell fate: a comparism between plants and animals
MADS gene family
HOX gene family
|
Pristupačnost
