About employee

Karlo Miškec, mag. biol. mol.

Title: Expert associate
Location: Borongajska cesta 83H, BIOCentar
E-mail: E-mail
Personal web page: https://www.linkedin.com/in/karlo-mi%C5%A1kec-324392173/?originalSubdomain=hr
Department: Division of Molecular Biology
mr.sc. graduation year:2019
Employed in this institution since:2020

Teaching

undergraduate

Consultations

By arrangement, contact by email.

Biography

I'm a molecular biologist with two years of experience in working with plant tissue culture, bacterial and yeast strains. During my internship and Master's thesis internship I've mostly worked with recombinant protein expression and have mastered molecular methods such as Western blot, pull down, yeast two hybrid, X-Gal test, PCR, molecular cloning, optimizations of protein expression and solubility and other methods. I have great experience in plant tissue cultivation and manipulation as well as in bacterial and yeast cultivation.

Currently I am working as a PhD student in Laboratory of Genetics and Epigenetics under the mentorship of Ph.D, assoc. prof. Aleksandar Vojta where I am researching the mechanism of IgG Fc chain glycosylation pathway and potential GWAS hits for glycosylation with epigenetic CRISPR/dCas9 modular system in suspension stable monoclonal cell lines HEK293-FreeStyle with dCas9/VPR or dCas9/KRAB domains stably integrated in cells genome as well as in suspension cell line LCL. Currently we are developing a new in vivo system of gene manipulation with CRISPR/dCas9 system in mouse strains C57BL/6 with lentiviral technology.

List of select publications

1. A Transient Expression System with Stably Integrated CRISPR-dCas9 Fusions for Regulation of Genes Involved in Immunoglobulin G Glycosylation

Authors:
Anika Mijakovac, Karlo Miškec, Jasminka Krištić, Vedrana Vičić Bočkor, Vanja Tadić, Maria Bošković, Gordan Lauc, Vlatka Zoldoš, Aleksandar Vojta

Publication date: 2022/1/12

Journal: The CRISPR Journal

Publisher: Mary Ann Liebert, Inc., publishers

2. Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism

Authors
Anika Mijakovac, Julija Jurić, Wendy M Kohrt, Jasminka Krištić, Domagoj Kifer, Kathleen M Gavin, Karlo Miškec, Azra Frkatović, Frano Vučković, Marija Pezer, Aleksandar Vojta, Peter A Nigrović, Vlatka Zoldoš, Gordan Lauc

Publication date:  2021/5/25

Journal: Frontiers in immunology

Volume: 12

Pages: 1922

3. BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism

Authors:
Mateja Jagić, Tamara Vuk, Andreja Škiljaica, Lucija Markulin, Vedrana Vičić Bočkor, Mirta Tokić, Karlo Miškec, Genadij Razdorov, Siniša Habazin, Marko Šoštar, Igor Weber, Nataša Bauer, Dunja Leljak Levanić

Publication date:  2022/9/6

Journal: Plant Cell Reports

Publisher: Springer Berlin Heidelberg

Pages: 1-19

List of select projects

KK.01.1.1.04.0085, project "Genomic engineering and gene regulation in cell lines and model organisms by CRISPR/Cas9 technology - CasMouse" 20.12.2019. - 31.12.2022.

Group Leader: Full Professor, Vlatka Zoldoš