Introduction: thermodynamics in biochemistry. Molecular interpretation of thermodynamical principles. Thermodynamical properties of macromolecular solutions. Thermodynamics of equilibrium and nonequilibrium states. Reversible and irreversible processes. Mechanisms of endergonic processes in biological and biochemical systems. Metastability, chemical potential of functional groups in biochemical systems. Enzymatic complexes. Energetic potential in living cells. Thermodynamics of membrane transport. Stabilisation of protein structure. Stabilisation forces and interactions in process of protein folding. Structural and functional domains. Uncovalent interactions: strength, energy and distance dependence. Models for studding uncovalent interactions in biochemical systems, importance of solvation. Dynamics of proteins structure. Protein-ligand interactions and their thermodynamical properties. Analysis of experimental data. Equilibrium processes, equilibrium constants - measurements and interpretation. Energetics of enzyme catalysis, transition state theory, energy of activation. Influence of steric factors on enzyme catalysis. Conformational changes: molecular mechanisms, cooperativity, allosteric effects. Mechanisms of cooperativity. Negative cooperativity. Physiological importance of cooperativity. Examples.
description of instruction methods Lectures, seminars, consultations.
- OBVEZNA LITERATURA:
D. A. Harris: Bioenergetics at a Glance, Blackwell Scienece, Oxford 2005.
K.E. van Holde, W.C. Johnson, P.S. Ho: "Principles of Phsical Biochemistry", Pretince Hall, Upper Saddle River, USA, 1998.
Miller: "Essentials of chemical biology", J. Wiely, Chichester, 2008.
G.U. Nienhaus: "Protein-Ligand Interactions - Methods and Application", Human Press Inc., Totowa, New Jeresy, 2005.
R.M.J. Cotterill: "BIOPHYSICS (An Introduction)", J. Wiely, West Sussex, 2006.
M. Flögel: Scripta biochemica: Bioenergetika, Enzimska kataliza, FBF, Zagreb 1993-1996.
T. A. Waigh: "Applied Biophysics- A Molecular Approach for Physical Scientists", J. Wiely, West Sussex, 2007.
- DOPUNSKA LITERATURA:
revijalni članci i znanstvene monografije