1. Biochemical and biophysical characteristics of lipid molecules: triglycerides, fatty acids, cholesterol, cholesterol ester, phospholipids
2. Lipoproteins: composition, classification, biochemical/biophysical properties
3. Biogenesis and metabolism of triglyceride rich lipoproteins:Chilomikrons, VLDL,; the role of lipoprotein lipase (LPL), LPL-knock out mice-a model for human LPL-deficiency; why LPL-deficiency is lethal in mice but not in humans? How experimental gene therapy using recombinant LPL-adenovirus facilitates survival of LPL-deficient mice?
4. Biogenesis and metabolism of low density lipoprotein: Generation of LDL, LDL receptor, molecular mechanisms of regulation of LDL receptor synthesis; LDL-receptor deficiency in humans, mutations in LDL receptor and in PCSK9; drugs and approaches used for treatment of hypercholesterolemia: statins, LDL apheresis, gene therapy; new drugs in clinical trials: limitapide and mipomersen
5. HDL-its role in reverse cholesterol transport (RCT): the role of ABCA1 and ABCG1 in the first step of RCT; the role of SR-BI, LCAT and CETP in HDL maturation; the role of hepatic SR-BI for delivery of HDL associated cholesterol ester into liver
6. Pathogenesis of atherosclerosis-the role of LDL, HDL and macrophages; application of synthetic HDL (reconstituted HDL), apoAI mimetics and mutant apoAI in the treatment of atherosclerosis; experimental atherosclerosis
7. Endothelial lipase-modulator of abundance, structure and function of HDL
description of instruction methods Lectures, seminars, problem based learning,
description of course/module requirements
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