Chemical, biological and physical foundations of biochemistry. Protein composition and structure: amino acids, primary, secondary, tertiary and quaternary structure, denaturation and folding. Examples of protein structure and function: myoglobin and hemoglobin. Methods in protein research. Enzymes: basic concepts, kinetics (Michaelis-Menten model), inhibition. Catalytic strategies of enzmyes: chymotrypsin example. Regulatory strategies: allosteric regulation, isoenzymes, phosphorylation/dephosphorylation, proteolytic activation. Composition and function of cellular membranes. Nucleic acid structure and role in transmission of genetic information. DNA replication and repair. RNA synthesis and processing. Protein biosynthesis. Control of gene expression in prokaryotes and eukaryotes.
Seminars will comprise solving of the problem and numerical questions that accompany each lecture.
1. to specify and explain structure and function of major biological (macro)molecules (proteins, nucleic acids, lipids, carbohydrates)
2. to explain and interpret the structure-function relationship of biological (macro)molecules
3. to explain principles of enzyme catalysis (mechanisms and kinetics of enzyme reaction, regulatory strategies)
4. to specify and explain composition and function of cellular membranes
5. to show knowledge and understanding of basic biochemical processes involved in the flow of genetic information in prokaryotes and eukaryotes
6. to interpret structure and modes of action of enzymes and macromolecular complexes involved in the flow of genetic information and regulation of gene expression