LEARNING OUTCOMES:
1. Comprehend the importance of plant chemical substances and their potential for human health protection and disease treatment
2. Characterize the main groups of plant bioactive substances and identify them based on their chemical structure
3. Relate chemical structures of plant substances with plants that contain them
4. Understand the principles of herbal medicines effects on human health
5. Link action and potential risks of herbal preparations, alone or in combination with other vegetable and/or synthetic drugs
6. Create tea blends for treatment of specific ailments/diseases based on understanding the principle of each component effect.
COURSE DESCRIPTION:
1. Heterosides: phenolic (salicin, arbutin), cardiac (digitoksigenin, gitoksigenin, gitaloksigenin), saponins (escin, ginsenosides), flavonoids (referring to quercetin, its effects on cell cycle, on the synthesis of hsp proteins, proliferation and apoptosis of tumor cells, its antioxidative propertiesas as well as synergistic effects of quercetin with some cytostatica and its interaction with RNA, DNA and some enzymes in human cells) antraquinonic (frangulin, emodin), kumarinic (unsubstituited and condensed)
2. Alkaloids (hashish, opium, alkaloids from Atropa belladona);
3. Biogenic compounds in the tumour therapy (alkaloidis from Vinca, quercetin, propolis)
4. Biogenic imunostimulans and imunosupresives
Chemical structures of mentioned classes of plant bioactive substances as well as their in vitro and in vivo biological effects and potential risk in the therapy will be presented. Molecular mechanisms of their effects will be pointed out.
PRACTICUM (condensed schedule):
1. HPLC analysis of phenolic compounds
a) introduction to extraction methods
b) analysis implementation
c) preparation of calibration curves for determination of substances concentration
d) determination of substances concentration
e) isolation of the phenolic compounds of interest
2. Spectrophotometric determination of phenolic substances content
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Pristupačnost
