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Tumor Biology
Code: 47314
ECTS: 4.0
Lecturers in charge: prof. dr. sc. Nada Oršolić - Lectures
Lecturers: Anica Horvat Knežević - Practicum
Take exam: Studomat
Load:

1. komponenta

Lecture typeTotal
Lectures 30
Practicum 30
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
LEARNING OUTCOMES:
Students will be able to:
1. to explain the origin and development of tumors
2. to explain which are carcinogens, where there are carcinogens, the biotransformation of carcinogens, how to avoid or minimize the danger of their actions
3. Point to the mechanism of action of carcinogens in the process of tumor genesis
4. to explain the process of metastasis, the relationship of the tumor and host during the metastatic cascade
5. find out what factors facilitate and which hinder the process of metastasis formation
6. to explain the basic principles of chemotherapy, hormonal therapy, hyperthermia, phototherapy, immunotherapy, biotherapy
7. to describe new approaches in the treatment of tumors and metastases, such as gene therapy, nanoparticles, inhibition of angiogenesis, inhibition telomerazne activities
8. to describe the mechanisms of acquiring resistance to chemotherapy and the abolition of the resistance mechanisms
9. students willto applay some important methods for evaluating the effectiveness of therapy on tumor growth and metastasis
10. will create a design of experiments to evaluate host immune reactivity to the tumor and the possibility to enhance the host immune response
11. Students will to identify and analyze the positive and negative effects of therapy and how to minimize the adverse effects of therapy
12. critically examine and evaluate the importance of experiments, animal models in assessing the effectiveness of specific therapies

COURSE CONTENTS:
1.What is the tumor? The hallmark of tumor cells
2. Molecular biology of carcinogenesis and tumor development. Chemical, physical and biological carcinogenesis.
3. Carcinogens, Metabolic activation of chemical carcinogens, The fate of carcinogens in the body, Cocarcinogens, Endogenous carcinogenesis
4. Tumors of the disease genes, genetic and epigenetic factors, Oncogenes and tumor suppressor genes
5. Molecular pathophysiology of tumor transformation of tumor cells, genetic, and metabolic barrier imunonadzorna
6. Immunobiology of tumor
7. Growth and metastasis of tumors
8. Invasiveness of tumor pathogenesis and metastasis; Influence of target tissues to the formation of metastases
9. Metastatic tumor cells and the extracellular matrix; Factors mobility of tumor cells and metastasis, Angiogenesis, Role of metalloproteinases in tumor progression
10.Molecular genetics and regulation of tumor cell invasiveness and metastasis
11. Immune surveillance, avoidance mechanisms of tumor response
12. Principles of oncologic therapy
13. New approaches to diagnosis and treatment of metastases

EXERCISES:
1.Introduction to working with tumors - on the in vivo tumor models;
Work on cells in vivo:
a. Ehrlich ascites tumor
b. Mammary gland carcinoma (MCA)
2. Determination of the number of metastases in the lung in a CBA strain mouse
3. Monitoring growth of EAT tumors in Swiss albino mice and monitoring the survival of animals.
4. Determining the functional ability of macrophages from the abdominal cavity
5. Differential cell analysis of the abdominal cavity
6. Determination of cell viability in culture using the in vitro cytotoxicity test - MTS
7. Testing cytotoxic effects of different compounds on the culture of adherent cells using clonal cell growth method
Literature:
  1. Boranić M. i sur. Karcinogeneza suvremena gledišta o podrijetlu malignih tumora. Medicinska naklada, Zagreb, 2000.
  2. Steven T. Rosen MD. The biology of sceletal metastasis, 2004, Culwer Academic Press Eds. Keller ET. And Chung WK.
  3. Marko Turić, Krsto Kolarić , Damir Eljuga, Klinička onkokologija, Nakladni zavod Globus, 1996
  4. Knowles M, Selby P. Introduction to the cellular and molecular biology of cancer, Oxford Univ Press, Oxford, 2010
  5. Grabarević Veterinarska onkologija, 2002, DSK-FALCO, Zagreb
8. semester
Izborni predmeti biologija i /ili kemija - Mandatory studij - Biology and Chemistry Education
Consultations schedule: