1. komponenta

Lecture type	Total
Lectures	60
Practicum	45
Seminar	15

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE SUMMARY
Within this course, students will acquire broad knowledge of genetics, ranging from classical principles of inheritance to modern molecular approaches. Special emphasis will be placed on the fundamental concepts of Mendelian and cytoplasmic inheritance, as well as on epistasis, incomplete dominance, expressivity, and penetrance, and their application in analyzing gene function and interactions. Students will be introduced to the foundations of molecular and population genetics, microbial genetics, and gene mapping methods. The course will cover both the classical (forward genetics) approach to genetic research, based on random mutagenesis, and the reverse genetics approach, which starts from collections of sequenced mutants, as well as the design of genetic experiments. Particular attention will be devoted to the application of molecular methods in genetic research, including sequencing technologies and tools for gene expression analysis. Part of the course will focus on applied genetics, where students will gain insight into the principles of first- and second-generation genetic engineering (restriction endonucleases and CRISPR-Cas9 technology), as well as the development of genetically modified plants and animals.

PRACTICAL CLASSES
Each practical session is organized as a hands-on exercise in a specific area of genetics, typically carried out in pairs, followed by a written report. In addition, every session includes collaborative work on quantitative problem-solving tasks. Students will: analyze the segregation of phenotypic traits using model organisms such as the plant Arabidopsis thaliana and the animal Drosophila melanogaster; determine the relative distance between genes on a genetic map; prepare chromosome slides; determine whether two mutations are located in the same gene; cultivate a genetically modified plant; isolate genomic and plasmid DNA; amplify selected gene fragments from model organism genomes; digest plasmids with restriction endonucleases; and visualize genes using gel electrophoresis.

SEMINARS
Through seminar work, students deepen their understanding of selected topics introduced in lectures by analyzing scientific or science-popular articles and book chapters. Each topic comes with a list of suggested references, but students are expected to independently search for additional, up-to-date literature each year. Seminar results are presented in the form of a PowerPoint presentation. Evaluation is based on clarity of presentation, the structure and quality of the slides, and the ability to answer questions from peers and instructors.

1. Genetics: from genes to genomes, Glavna preporučena literatura. Udžbenik prati predavanja za većinu tema., M.L. Goldberg and L.H. Hartwell, McGraw Hill, 2021.
2. Introduction to genetic analysis, Ovaj udžbenik objašnjava genetičke fenomene kroz eksperimentalne primjere., A.J.F. Griffiths, Macmillan Learning, 2020.
3. Concepts of genetics, W.S. Klug, Pearson, 2019.
4. Essential genetics and genomics, D.L. Hartl, Jones&Bartlet Learnig, 2020.
5. Genetics: analysis and principles, R.J. Brooker, McGraw Hill, 2024.
6. Genetic analysis: an integrated approach, M.F. Sanders and J.L. Bowman, Pearson, 2019.
7. Genetics: a conceptual approach, B. A. Pierce, Macmillan Learning, 2020.

Enrollment :
Passed : Cell Biology

Mandatory course - Regular study - Molecular Biology