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Molecular Plant Pathology
Code: 83820
ECTS: 6.0
Lecturers in charge: izv. prof. dr. sc. Martina Šeruga Musić - Lectures
Lecturers: Martina Šeruga Musić - Seminar

Martina Šeruga Musić - Practicum
Take exam: Studomat
English level:

1,0,0

All teaching activities will be held in Croatian. However, foreign students in mixed groups will have the opportunity to attend additional office hours with the lecturer and teaching assistants in English to help master the course materials. Additionally, the lecturer will refer foreign students to the corresponding literature in English, as well as give them the possibility of taking the associated exams in English.
Load:

1. komponenta

Lecture typeTotal
Lectures 30
Practicum 30
Seminar 15
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
Expected learning outcomes:

Students should be able to:
1. Recognize the significance of plant pathogy and the diversity of their causing agents
2. Understand infectious disease cycles of plants and the mechanisms of plant host response
3. Understand the role and importance of molecular biology in plant pathogen, plant disease and plant-pathogens interaction research
4. Apply and implement different molecular biology techniques in detection and characterization of plant pathogens
5. Search scientific literature on given scientific topic
6. Analyze and present a scientific work

Course content broken down in detail by weekly class schedule (syllabus):

1. Introduction to plant pathology. The concept of plant disease. The causal agents - fungi, the oomycota, bacteria, viruses, other. The significance of plant diseases. The control of plant diseases. Molecular biology in plant pathology.
2. Fungal and Oomycetes diseases. Pathogenesis of diseases. Establishing of infection. Development of disease. Effectors.
3. Fungal and Oomycetes genetics. Mechanisms for generating genetic variation. Chromosome instability. Horizontal gene transfer. Role of transposable elements. Role of mycoviruses.
4. Bacterial diseases. Pathogenesis. Establishing of infection. Bacterial-bacterial communication - quorum sensing. Penetration to plant hosts.
5. Bacterial diseases - determinants of host specificity. Concept of effector biology - avirulence factors and toxins; elicitors. Type III secretion mechanism. Pathogenicity islands. The role of plasmids.
6. Phytoplasmas and spiroplasmas as plant disease causing agents. Diversity and reductive evolution. Pathogenicity strategies.
7. Plant viruses as plant pathogens. Structure and replication.
8. Plant viruses- movement and interaction with plants.
9. Pathogens effects on plant physiological functions. Effects of environmental factors on development of infection diseases of plant.
10. Resistance mechanisms in plants. Plant immunity. Classical concept of resistance. Preformed resistance. Indiced defences. Gene-to-gene resistance.
11. Signalling in plat disase resistance mechanisms. PAMP- triggered immunity (PTI).. Effector triggered immunity (ETI).
12. Genetics and molecular epidemiology of plant diseases.
13. Molecular dignostics of plant diseases.
14. Application of molecular biology to conventional disease control strategies.
15. Transgenic approaches fro crop protection. Pathogen-derived resistance. Platibodies. Overexpressing defence genes. Expression of vaccines in plants.

Laboratory practicals (exercises):
1. Isolation of total nucleic acid (TNA) and determination of TNA concentration (4 h).
2. Isolation and SDS-PAGE of total proteins (4 h)
3. Multilocus sequence (MLST) analyses -PCR amplification of different pathogen gene regions followed by RFLP and sequencing (6 h).
4. Pathogen detection by real-time PCR (2 h).
5. SSCP analyses for detection of molecular variability (6 h)
6. Southern blot analyses (6 h)
7. Phylogenetic sequence analyses (2 h)


Seminars:
1. Selection of seminar topics from the molecular plant pathology field (2 h)
2. Literature search (6 h)
3. Oral presentation of seminars (7 h)
Literature:
  1. Juretić N (2002) Osnove biljne virologije. Školska knjiga, Zagreb
    Prescott LM, Harley JP, Klein DA (2002) Microbiology. McGraw-Hill, Boston
  2. Dickinson M (2003) Molecular Plant Pathology. BIOS Scientific Publishers, London
    Agrios GN (2005) Plant Pathology (Fifth Edition). Academic Press, New York
  3. Wiley J, Sherwood L, Woolverton C (2013) Prescott's Microbiology, 9th edition, McGraw-Hill, Boston
  4. noviji odabrani originalni znanstveni i revijalni članci
1. semester
Izborni predmeti - Mandatory studij - Molecular Biology
Consultations schedule: