1. komponenta

Lecture type	Total
Lectures	60
Practicum	45

Course objectives:
The aim of the course Microbiology is (i) acquiring knowledge on basic concepts in virology and prokaryote microbiology and (ii) mastering fundamentals of molecular biology on viruses as models.

COURSE CONTENT
LECTURES (bacteriology; virology):
1. History of Microbiology I ; Historical overview of virology
2. History of Microbiology II ; Viruses ? biological properties and general significance
3. Morphology and size of prokaryotic cells I; Viral genomes
4. Morphology and size of prokaryotic cells II; Replication strategies of viral genomes
5. Structure of bacterial cell !; Viral proteins and virion structure
6. Structure of bacterial cell II; Viral cycle
7. Dynamics of bacterial growth and metabolism !; Detection and characterisation of viruses and subviral pathogens
8. Dynamics of bacterial growth and metabolism II; Virus evolution
9. Biogeochemical cycles I; Pathogenesis of viral diseases
10. Biogeochemical cycles II; Vaccines
11. Bacterial classification I; Antiviral therapy
12. Bacterial classification II; Bacteriophages and their pathogenic potential
13. Clinically relevant bacteria I; Oncogenic viruses, HPV
14. Clinically relevant bacteria II; Viral ecology and epidemiology
15. Significant discoveries in microbiology this year; Subviral pathogens - types and biological features

PRACTICAL LABORATORY EXERCISES (bacteriology; virology):
1. Preparation of nutrient media; Working methods and precautions in the virology laboratory
2. Throat swab; Methods of cultivation of plant viruses
3. Environmental swab; Experimental viral infection of plants
4. Determining bacterial counts by CFU method; Recognition of virus infection symptoms on experimentally infected plants
5. Testing antibiotic susceptibility by disc diffusion method and determination of MBC value; Virus inclusion bodies
6. Isolation of pure (axenic) bacterial culture and cryo storage (Microbank); Experimental viral infection of bacteria
7. Bacteriological analysis of potable water; Quantification of bacteriophages
8. Cultivation of Clostridium spp.; Serological methods in virus research
9. Cultivation of nitrogen-fixing bacteria; Serological identification and quantification of viruses
10. Identification of bacteria by MALDI-TOF analysis; Virus purification
11. Biochemical tests, KOH, catalase, oxidaze tests; Quantitative and qualitative analysis of purified viruses
12. Gram staining; Laboratory cultivation of animal viruses
13. Staining of bacterial endospores; Methods for the production of attenuated vaccines
14. Staining of bacterial capsules; Virus propagation in embryonated chicken eggs
15. Ziehl-Nielsen stain; Summarisation of the results

1. Prezentacija predavanja on line, Biološki odsjek, PMF.
2. Praktikum iz bakteriologije, Stilinović B., Hrenović J., Kugler, Zagreb, 2009.
3. Priručnik za vježbe iz opće mikrobiologije, Antolović i sur., Hrvatsko mikrobiološko društvo, Zagreb, 2016.
4. Opća mikrobiologija, Duraković S., Nacionalna i sveučilišna biblioteka, Zagreb, 1996.
5. Osnove biljne virologije, Juretić, N., Školska knjiga, Zagreb, 2002.
6. Virologija, Presečki, V. i sur., Medicinska naklada, Zagreb, 2003.
7. Virology - Principles and Applications, Carter JB, Saunders VA, John Wiley and Sons, New York, 2011.

Mandatory course - Regular study - Biology