The aim of this course is to acquire knowledge about the biological activity (mechanisms of action, antioxidant and cytotoxic activity as well as interactions with nucleic acids) of phytochemicals and practical skills in the assessment of different biological activities of extracts and/or compounds isolated from plants.
LEARNING OUTCOMES:
- identify and classify phytochemicals
- evaluate the selection of the appropriate method for a particular biological activity
- distinguish between in vitro and in vivo methods for determining biological activity
- apply the acquired knowledge in the preparation of a poster presentation, design and present it
- evaluate and critically analyze data from the field of traditional medicine
- determine how to concisely and in simple way present results and scientific facts in front of an audience
LECTURES
1.-3. Classes of phytochemicals and mechanism of their action
4.-7. Metabolism of phytochemicals in humans
8.-9. Methods of extraction, isolation and characterization of active substances from plant extracts
10.-11. Assessment of antioxidant activity by in vitro and in vivo methods
12.-13. Evaluation of cytotoxic and antitumor activity by in vitro and in vivo methods
14.-15. Interactions of extracts and/or compounds with nucleic acids
SEMINARS
1., 3. Selection of an original scientific paper from the field with a short education on the structure and understanding of a scientific paper on an example
4., 9. Critical evaluation of selected scientific work and poster preparation (manual creation) and independent work
10., 12. Short oral presentation of work (poster) discussion
13., 14. Tips for preparing a successful PP presentation with examples
15., 22. Preparation of presentations in the field of traditional medicine; usage review; independent work
23., 27. Workshop; production of products from selected topics of traditional medicine
28., 30. Presentation of the topic in a scientific and popularizing manner and discussion with questions from the audience
PRACTICAL WORK
1.-3. Preparation of extracts using several solvents
4.-6. Determination of total phenols and flavonoids
7.-8. Determination of antioxidant activity by DPPH and ABTS methods
9.-10. Determination of cytotoxicity by the crystal violet method or similar method
11.-13. Investigation of interactions of compounds with DNA and RNA using spectroscopic methods (UV and Vis, fluorimetry, circular dichroism)
14.-15. Statistical analysis and presentation of results
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Pristupačnost
