Small Angle X-ray Scattering, SAXS (angles 0.1-10 degrees), is a method in the study of non-crystalline materials. Characteristic scale of the studied structure and the scattering angle are inversely proportional, thus SAXS provides information on relatively larger scales, 1-100 nm. Scattering intensity distribution is a function of the electron density in the sample and thus may provide information on size, shape, orientation and distribution of the building elements (macromolecules, colloidal particles and other supramolecular structures) of the sample. We will present the application of SAXS in the study of the conformation of polyelectrolytes. We will use DNA as an example of a semi-rigid biopolyelectrolyte.
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