1. komponenta

Lecture type	Total
Lectures	30
Practicum	30

* Load is given in academic hour (1 academic hour = 45 minutes)

THE COURSE DESCRIPTION
The course presents basic theoretical and practical knowledge on animal and plant cell culture and gives and introduction to basic biological mechanisms that regulate cell cycle, signaling, processes of differentiation, apoptosis, senescence and tumorigenesis.
THE AIM OF THE COURSE
The aim of the course is to enable students to describe possible applications of the animal and plant cell culture in science and biotechnology,to analyze positive and negative features of different culturing techniques, to prepare nutrient media and manipulate with animal and plant cells, as well as use methods of plant regeneration using protocols.
COURSE CONTENT:
LECTURES
ANIMAL CELL CULTURE
1. INTRODUCTION INTO ANIMAL CELL CULTURE AND CONDITIONS FOR CELL GROWTH; definition of animal cell culture, its use, comparison between cell culture and model organisms, hystory of cell culture, types of cell media and surfaces, contamination, cell freezing, cell lines types, growth curve.
2. PRIMARY CELL CULTURE; preparation of primary culture, methods of separation of different cell types, evolution of the cell line
3. CELL QUANTITATION; methods of cell quantitation, cell counting, determination of the cell viability by differential staining, flow cytometry.
4. CELL AUTHENTICATION AND CHARACTERIZATION; methods of cell authentication and characterization
5. PRINCIPLES OF CELL DIFFERENTIATION AND SIGNALLING; principles of cell differentiation and signallization, methods of cell differentiation, cell growth in 3D systems
6. CELL CYCLE AND METHODS OF CELL SYNCHRONIZATION; cell cycle regulation and cell synchronization in the culture, methods of synchronicity testing
7. MODERN METHODS IN SCIENCE; new techniques in cell culture, cell fusion methods of plasmid transfection, microscopy
8. TUMORIGENESIS AND SENESCENCE; basic characteristics of tumor cell growth and senescence

EXERCIZES
1. INTRODUCTION WITH CELL GROWTH CONDITIONS IN CULTURE; introduction into laboratory and basic techniques of cell culture, trypsinization and cell counting, seeding cells
2. GROWTH CURVE; seeding cells for growth curve and analysis of cell growth in culture by counting, calculation of the duplication time
3. PRIMARY CULTURE; preparation of fibroblast culture from mouse skin. Explantation technique and preparation of cell suspension by trypsinization
4. ANALYSIS OF CYTOTOXICITY; analysis of influence of chemotherapeutics on the cell growth, growth curve obtained by counting the cells
5.ANALYSIS OF CELL SURVIVAL; analysis of influence of chemotherapeutics on the cell growth, survival curve obtained by counting colonies
6. CELL SYNCHRONIZATION; methods of mitotic selection

Izborni predmeti - Regular study - Biology