|Lokacija:||Borongajska cesta 83H, BIOCentar|
|Osobna stranica na Webu:||https://www.linkedin.com/in/karlo-mi%C5%A1kec-324392173/?originalSubdomain=hr|
|Zavod/služba:||Zavod za molekularnu biologiju|
|Na zavodu od:||2020.|
Po dogovoru, kontaktirati mailom.
I'm a molecular biologist with two years of experience in working with plant tissue culture, bacterial and yeast strains. During my internship and Master's thesis internship I've mostly worked with recombinant protein expression and have mastered molecular methods such as Western blot, pull down, yeast two hybrid, X-Gal test, PCR, molecular cloning, optimizations of protein expression and solubility and other methods. I have great experience in plant tissue cultivation and manipulation as well as in bacterial and yeast cultivation.
Currently I am working as a PhD student in Laboratory of Genetics and Epigenetics under the mentorship of Ph.D, assoc. prof. Aleksandar Vojta where I am researching the mechanism of IgG Fc chain glycosylation pathway and potential GWAS hits for glycosylation with epigenetic CRISPR/dCas9 modular system in suspension stable monoclonal cell lines HEK293-FreeStyle with dCas9/VPR or dCas9/KRAB domains stably integrated in cells genome as well as in suspension cell line LCL. Currently we are developing a new in vivo system of gene manipulation with CRISPR/dCas9 system in mouse strains C57BL/6 with lentiviral technology.
Anika Mijakovac, Karlo Miškec, Jasminka Krištić, Vedrana Vičić Bočkor, Vanja Tadić, Maria Bošković, Gordan Lauc, Vlatka Zoldoš, Aleksandar Vojta
Publication date: 2022/1/12
Journal: The CRISPR Journal
Publisher: Mary Ann Liebert, Inc., publishers
Anika Mijakovac, Julija Jurić, Wendy M Kohrt, Jasminka Krištić, Domagoj Kifer, Kathleen M Gavin, Karlo Miškec, Azra Frkatović, Frano Vučković, Marija Pezer, Aleksandar Vojta, Peter A Nigrović, Vlatka Zoldoš, Gordan Lauc
Publication date: 2021/5/25
Journal: Frontiers in immunology
Hrvatska udruga genetičkih inženjera - HUGI.
KK.01.1.1.04.0085 Genomsko inženjerstvo i genska regulacija u staničnim linijama i modelnim organizmima tehnologijom CRISPR/Cas9 - CasMouse 20.12.2019. - 31.12.2022.
Voditelj projekta: prof.dr.sc Vlatka Zoldoš