1. komponenta

Lecture type	Total
Lectures	30
Practicum	15

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE CONTENT
LECTURES
1. Introductory into radiobiology: what it represents, with the emphasis put on harmful biological effects, review of major scientific achievements, radiation types.
2. Isotopes: stable and unstable, application of radioisotopes in medical diagnostics and therapy.
3. Radioactivity: measuring units, absorbed dose, dose rate, exposure, quality assurance, linear energy transfer (LET).
4. Environmental radiation sources: natural and man-made sources, application of radiation sources in medical and diagnostics and therapy, radiation (external and internal).
5. Measurements of ionizing radiation: measuring devices (dosimeters, counters, track visualization devices, personal dosimetry).
6.7. Physical properties of ionizing radiation: direct and indirect interaction of radiation energies with biological media, interaction with biological macromolecules.
8.9. Radio sensitivity of cells: effects on intracellular structures and organelles, changes in DNA molecule.
10. Survival curves: viruses, bacterial, mammalian cells, Target theory.
11. Radio sensitivity of chromosomes: mechanisms of DNA-damage, changes of chromosomal structure, biological consequences.
12. Tissue radio sensitivity: delays effects of radiation (type of acute radiation syndromes).
13. Modificators of radio sensitivity: chemical radio protectors, radiosensibilizators, mechanism underlying radio sensibility, detection and measurements.
14. Bio monitoring: exposure of general and occupationally exposed population of the Republic of Croatia.
15. Radiofrequency-microwave radiation.

PRACTICAL TRAINING
Introduction
Introduction to instruments for detection and measurement of ionizing radiation (GM detector, scintillation device, thermoluminescent dosimeters).
In different experimental models: in vivo and ex vivo determination of the biological effects of ionizing radiation. The analysis of cytological slides: Analysis of chromosomal aberrations, micronucleus test analysis, micro gel electrophoresis of single cells, Cytotoxicity assay

1. Down SB, Tilson ER. Practical Radiation Protection and Applied Radiobiology, 2nd ed. Saunder WB Company, 1999, Toronto
   
   Polk C, Postow E. Biological Effects of Electromagentic Fields,2 nd ed. CRC Press, 1996, Washington, USA.
   
   Nataraajn AT. 2002. Chromosome aberrations:past,present and future. Mut res 504:3-16-
   
   Fenech M. 2006. Cytokinesis-block micronucleus assay evolves into a cytome assay of chromosmal instability, mitotic dysfunction and cell death. Mut res 600 58-66
   
   Collins A et al. 2008. The comet assay:topical issues. Mutagenesis 23;143-151.
2. IAEA; Biological Radiation Effects,Springer Verlag.1990.
   Slater RJ. 1990. Radioisotopes in Biology,IRL Press Oxford

MODUL: BIOLOŠKA ZAŠTITA OKOLIŠA - Regular study - Environmental Sciences

MODUL: BIOLOŠKA ZAŠTITA OKOLIŠA - Regular study - Environmental Sciences

Izborni predmeti - Regular study - Molecular Biology