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Cell and Molecular Biology
Code: 40929
ECTS: 5.0
Lecturers in charge: prof. dr. sc. Biljana Balen - Lectures
prof. dr. sc. Višnja Besendorfer - Lectures
Lecturers: Renata Biba - Practicum
Take exam: Studomat
English level:

1,0,0

All teaching activities will be held in Croatian. However, foreign students in mixed groups will have the opportunity to attend additional office hours with the lecturer and teaching assistants in English to help master the course materials. Additionally, the lecturer will refer foreign students to the corresponding literature in English, as well as give them the possibility of taking the associated exams in English.
Load:

1. komponenta

Lecture typeTotal
Lectures 30
Practicum 45
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
LEARNING OUTCOMES:
1. Recognize, define and describe structure of eukaryotic cell and compare it with the structure of simpler prokaryotic cell.
2. Describe and explain processes which take place in certain cellular compartments and connect them with functioning of the entire cell.
3. Identify and describe each phase of cell division of somatic and germ cells.
4. Apply techniques of light and electron microscopy and use basic techniques of molecular biology.
5. Analyse, summarise and interpret information obtained on the basis of the written text (book chapter or scientific article).

COURSE CONTENT:
During this course students will get an integral knowledge about chemical and biological origin of life as well as on cell structure, molecular organisation and function of cell organelles. The special attention is given to the practical part of the course where students get the basic skills in light microscopy and cell fractionation and acquire basic techniques of molecular biology.

LECTURES
1. Chemical composition of the cell
2. Methods in cell biology; Light and electron microscopy, Cell fractionation and centrifugation; Protein electrophoresis and chromatography; Comet test; Polymerase chain reaction
3. Cell theory and origin of the first cells; Basic plan of cellular organisation; Prokaryotic and eukaryotic cell; Differences between eukaryotic cells (plant and animal cell)
4. Biomembranes: biomembrane organisation; Fluid mosaic model; Lipid bilayer; Membrane proteins and carbohydrates; Membrane transport
5. Mitochondria: Organisation and function; inner membrane and its role in energy conversion (oxidative phosphorylation); Biogenesis and origin of mitochondria; Endosymbiotic theory; Mitochondrial genome
6. Plastids: Plastid; Plastid genome; Chloroplast structure and ultrastructure; Thylakoid membranes and energy conversion; Peroxisome structure and function
7. Endoplasmic reticulum: Organisation and function; Rough ER and cotranslational protein import; Smooth ER and lipid synthesis; Vesicular transport of molecules
8. Golgi complex: Organisation and function; Biochemical and functional compartmentalization of Golgi complex; Chlatrin coated vesicles; Lysosomes and vacuoles; Protein secretion
9. Cytoskeleton: Organisation and function; Microfilaments; Intermediate filaments; Microtubules; Cilia and flagella
10. Nucleus: Organisation and function; Nuclear envelope; Chromatin and chromosomes; From DNA to chromosomes; Synthesis of ribosomal RNA
11. Cell cycle; Mitosis and cell cycle control; Endomitosis; Polytene chromosomes; C-mitosis
12. Meiosis; Crossing over; Genetic recombination
13. DNA structure and replication
14. Transcription and Translation

PRACTICAL WORK
1. Microscopy
2. Cell structure and basic organisational cell types
3. Biomembranes: indirect observations
4. Plastids
5. Nucleus. Mitosis
6. Endomitosis. Polytene chromosomes and C-mitosis
7. Meiosis I: section preparation and microscopy
8. Meiosis II: problem solving
9. Isolation of nuclei and DNA fibres
10. Isolation of genome DNA from plant material by home-made method
11. Isolation of genome DNA from transgenic plant Arabidopsis thaliana and plasmid DNA from laboratory strain of bacterium Escherichia coli
12. PCR, electrophoresis and DNA restriction analysis
Literature:
  1. Molecular Biology of the Cell. 5th ed. Alberts, Bruce i sur. New York: Garland Publishing; 2008.
  2. The Cell - A Molecular Approach. 2nd ed. Cooper, Geoffrey M. Sunderland (MA): Sinauer Associates, Inc; 2000.
  3. Stanica, molekularni pristup. Cooper, Geoffrey M - hrvatsko izdanje, Zagreb: Medicinska naklada; 2004.
  4. Krsnik-Rasol M, Besendorfer V, Jelenić S, Balen B, Malenica N, Peharec P. PRAKTIKUM IZ STANIČNE I MOLEKULARNE BIOLOGIJE, interna skripta i obrasci, http://www.pmf.unizg.hr/biol
3. semester
Izborni predmeti - Mandatory studij - Environmental Sciences
Consultations schedule: