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Transplantation Immunology
Code: 45057
ECTS: 9.0
Lecturers in charge: prof. dr. sc. Zorana Grubić - Lectures
Lecturers: Zorana Grubić - Seminar

Zorana Grubić - Practicum
Marija Maskalan - Practicum
Katarina Štingl - Practicum
Take exam: Studomat
English level:

1,0,0

All teaching activities will be held in Croatian. However, foreign students in mixed groups will have the opportunity to attend additional office hours with the lecturer and teaching assistants in English to help master the course materials. Additionally, the lecturer will refer foreign students to the corresponding literature in English, as well as give them the possibility of taking the associated exams in English.
Load:

1. komponenta

Lecture typeTotal
Lectures 30
Practicum 45
Seminar 15
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
EXPECTED LEARNING OUTCOMES

1. Gain basic knowledge about immune processes that occur during transplantation of solid organs, tissues and hematopoietic stem cells
2. Gain knowledge about current advances as well as limitations in the field of transplantation immunology and medicine
3. Gain knowledge about Major Histocompatibility Complex in humans (HLA system) and its role in the transplantation processes
4. Learn how to perform the methods for molecular typing of HLA genes, serological typing method as well as HLA antibody detection
5. Gain knowledge about NK cells and KIR receptors and their role in transplantation reaction, application and typing methods of KIR genes; learn how to perform the methods for KIR gene typing
6. Gain knowledge about Minor histocompatibility antigens in humans, their role in transplantation reaction, application and typing methods; learn how to perform the methods for Minor gene typing
7. Gain knowledge about the estimation of patients chimeric status following the hematopoietic stem cell transplantation, prognostic value of the test and methods used for that purpose; learn how to perform the microsatellite loci analysis (PCR-STR method) for the purpose of chimerism monitoring
8. Gain knowledge about the transplantation program in Croatia and worldwide

COURSE CONTENT:

LECTURES
1. Cell, tissue and organ transplantation: history, application, types of transplantation
2. Cell, tissue and organ transplantation: immunobiological, surgical, ethical and legal issues
3. Immune system: role, organs (primary, secondary), cells (lymphocytes, granulocytes, antigen presenting cells), immunity (innate, acquired, active, passive), immune reaction (cellular, humoral)
4. Major Histocompatibility Complex (HLA system): main characteristics, role, location, polymorphism, linkage disequilibrium, products, tissue distribution, crossing-over, nomenclature, application
5. Molecular structure of HLA region (class I HLA region, central region, class II HLA region), HLA class I and class II genes (structure, role), HLA class I and class II molecules (structure, role)
6. Methods for HLA gene and molecule typing and HLA antibody detection
7. Non-classical HLA molecules (HLA-E, -F, -G), role, application
8. NK cells and KIR receptors (role, applications, nomenclature, importance)
9. Transplantation reaction, host versus graft reaction, graft versus host reaction
10. Kidney transplantation (immune reaction, limitating factors, impact of HLA genes compatibility between recipient and donor on transplantation outcome, organ allocation)
11. Transplantation of other solid organs (liver, heart, pancreas)
12. Hematopoietic stem cell transplantation (immune reaction, limitating factors, donor choice and probability of finding a donor, advantages and disadvantages of different hematopoietic cells sources)
13. Chimerism: application, importance, prognostic value, methods of detection
14. Waiting lists for solid organ transplantation, international transplantation organizations (Eurotransplant, Bone Marrow Donors Worldwide), and national organizations (Croatian Bone Marrow Donor Registry)

EXERCISES

1. Cell separation from peripheral blood
2. Methods of cell storage
3. DNA isolation
4. HLA gene typing using polymerase chain reaction with sequence specific primers (PCR-SSP)
5. Electrophoresis on an agarose gel
6. Analysis of HLA gene typing results using a computer software SCORE
7. HLA gene typing using polymerase chain reaction and hybridization with specific probes attached on microspheres (PCR-SSO)
8. Analysis of HLA gene typing results using a computer software Fusion
9. HLA class I molecule typing (mycrolymphocytotoxicity test MLCT)
10. Determination of panel reactive HLA antibodies in sera (%PRA)
11. Cross-match reaction (CM)
12. Determination of HLA phenotype, genotype and genealogy
13. Searching for unrelated donor in Bone Marrow Donors Worldwide and Croatian Bone Marrow Donors Registry
14. KIR gene typing using polymerase chain reaction with sequence specific primers
15. Minor genes typing using polymerase chain reaction with sequence specific primers
16. Microsatellite loci analysis using polymerase chain reaction with sequence specific primers (PCR-STR) for chimerism status determination in patients following the hematopoietic stem cell transplantation
17. Analysis of microsatellite results using a computer software AlleleLocator


SEMINARS

Each student will get a scientific article from the transplantation immunology field, which the student will present to his colleagues in the form of a short lecture. The article selection will be made in such way that the latest developments and accomplishments in the transplantation immunology field are presented.
Literature:
  1. 1. Andreis I, Batinić D, Čulo F, Grčević D, Marušić M, Taradi M, Višnjić D. Imunologija. Zagreb: Medicinska naklada, 2004.

    2. Marsh S.G.E., Parham P., Barber L.D. The HLA facts book. London: Academic Press, 2000.

    3. Mehra NK, Kaur G, McCluskey J, Christiansen FT, Claas FHJ. The HLA Complex in Biology and Medicine. London: Jaypee Brothers Medical Publishers (P) Ltd, 2010.
2. semester
Izborni predmeti - Mandatory modul - Physiology and Immunobiology
Consultations schedule: