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Immunochemistry and Immunochemical methods

Code: 152837
ECTS: 5.0
Lecturers in charge: dr. sc. Ruža Frkanec - Lectures
Take exam: Studomat
Load:

1. komponenta

Lecture typeTotal
Lectures 10
Seminar 5
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
Purpose and characteristics of laboratory testing
Chemical and physicochemical methods (chromatography, filtration, electrophoresis)
Basic interactions Ag-Ab (structure, binding, affinity, avidity)
Immunization (immunogens, haptens)
Immunoanalytical methods (agglutination and precipitation, radial and double diffusion, immunoelectrophoresis, immunoblotting)
Immunoassays-competitive and non-competitive (radioimmunoassay, enzyme-immunoassay, fluorometric immunoassay)
Literature:
  1. OBVEZNA LITERATURA:
    Richard Coico, Geoffrey Sunshine: Immunology - a Short Course, 7th Edition., Wiley&Blackwell, New York 2015.
    Immunology, 8th Edition, Editors: David Male, Jonathan Brostoff, David Roth, Ivan Roitt, Saunders, 2013.
    T. Chard: An Introduction to Radioimmunoassay and Related Techniques, Volume 6, 5th Edition, Elsevier, Amsterdam 1990.
    Tijssen P. Practice and Theory of Immunoassays, 8th edition, R.H. Burdon, P.H. van Knippenberg Editors, Elsevier, Amsterdam, The Netherlands, 1993.
    E. Harlow, D. Lane: Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory, 1988.
    E. D. Day: Advance Immunochemistry, 2nd Edition, Wiley-Liss, New York 1990.
    N.C. Price (ur.): Proteins, The Labfax series, BIOS Scientific Publishers, Academic Press, New York 1996.
    R. Edwards (Ed.): Immunoassays, Essential Data, Wiley, New York 1996.
    E. P. Diamondis, T. K. Christopoulos (Eds.): Immunoassay, Academic Press, San Diego 1996.
    J.P. Gosling: Immunoassays: A Practical Approach (The Practical Approach Series), Oxford University Press, USA, 2000.
    R.A. Goldsby, T.J. Kindt, B.A. Osborne, J. Kuby: Kuby Immunology, 6th Edition, W.H. Freeman and Company, New York 2007.
    C.A. Janeway, P. Travers, M. Walport, M. Schlomchik: Immunobiology The Immune System in Health and Disease: 6th Edition, Garland Publishing, New York, 2004.
    Imunologija (I. Andreis, D. Batinić, F. Čulo, D. Grčević, M. Marušić, M. Taradi, D. Višnjić), 6. izdanje, Medicinska naklada, Zagreb, 2004.
    Abul K. Abbas Andrew H. Lichtman Shiv Pillai, Stanična i molekularna imunologija, 8. izdanje, Medicinska naklada, Zagreb, 2018.
  2. DOPUNSKA LITERATURA:
    revijalni članci i znanstvene monografije
Consultations schedule: