Department of...
 
Biochemistry...
 
Biochemistry Laboratory
 
Code: 40860
ECTS: 2.0
Lecturers in charge: doc. dr. sc. Marko Močibob - Lectures
Take exam: Studomat
Load:

1. komponenta

Lecture typeTotal
* Load is given in academic hour (1 academic hour = 45 minutes)
Description:
COURSE CONTENT:
Potentiometric titration of amino acids. Determination of alcohol-dehydrogenase kinetic parameters for ethanol. Inhibition of alcohol-dehydrogenase activity. Size-exclusion chromatography of biological macromolecules. Size-exclusion chromatography of hemoglobin. Polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE). Electrophoresis of DNA on the agarose gel. Preparation of plasmid DNA from transformed bacterial cells.

LEARNING OUTCOMES:
1. to specify and explain basic rules for safe work in biochemical laboratory
2. to independently perform experiments using standard biochemical techniques and methods
3. to use basic biochemical equipment and instruments
4. to appropriately handle biological macromolecules in order to avoid their denaturation and inactivation
5. to specify and explain methods for purification and analysis of proteins and nucleic acids
6. to perform enzyme kinetics experiments, including inhibition of enzyme reaction, and to determine kinetic parameters
7. to apply mathematical methods for data analysis
8. to graphically show results of experiments
9. to critically evaluate experimental results
10. to write a report on laboratory exercises
Literature:
  1. OBAVEZNA LITERATURA:
    Interna skripta za Praktikum iz biokemije (13. radno izdanje), Zavod za biokemiju, PMF, Zagreb, 2007.
    Relevantna poglavlja iz udžbenika L. Stryer, J. Berg i J. Tymoczko, BIOKEMIJA (6. izd), Školska knjiga, 2013. (prijevod na hrvatski jezik)
    ili
    Relevantna poglavlja iz udžbenika J. M. Berg, J. L. Tymoczko i L. Stryer, BIOCHEMISTRY (7. izd.), W. H. Freeman & Co, New York, 2012.
  2. DOPUNSKA LITERATURA:
    D. Voet i J. G. Voet, BIOCHEMISTRY (4. izd.), Wiley, New York, 2011.
    D. L. Nelson i M. M. Cox, LEHNINGER PRINCIPLES OF BIOCHEMISTRY (6. izd.), W. H. Freeman & Co, New York, 2013.
Prerequisit for:
Enrollment :
Enrolled : Biochemistry
Passed : Cell Biology
6. semester
Mandatory course - Mandatory studij - Biology and Chemistry Education
Consultations schedule:
News - Archive
 
arrowl Return

Results 0 - 0 of 0
Page 1 of 0
Results per page: 
No news!
Repository
 
Repository is empty
Poll
 
No polls currently selected on this page!

 

 

Sva prava pridržana. Kemijski odsjek Prirodoslovno-matematičkog fakulteta
Sveučilište u Zagrebu