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Basic Biochemistry Laboratory

Code: 196123
ECTS: 2.0
Lecturers in charge: Morana Dulić, Assistant Professor, PhD
Marko Močibob, Assistant Professor, PhD
Lecturers: Practicum:
Morana Dulić, Assistant Professor, PhD
Marko Močibob, Assistant Professor, PhD
Petra Modrušan, mag. chem.
Take exam: Studomat
Load:

1. komponenta

Lecture typeTotal
Practicum 30
Description:
Potentiometric titration of amino acid. Determination of kinetic parameters of alcohol dehydrogenase for substrate ethanol. Inhibition of activity of alcohol dehydrogenase. Gel-filtration of hemoglobin.
SDS polyacrylamide gel electrophoresis of proteins. Isolation of plasmid DNA from transformed bacteria.
Agarose gel electrophoresis of plasmid DNA.
Literature:
  1. Praktikum iz biokemije (19. radno izdanje), Zavod za biokemiju, PMF, Zagreb, 2020., Interna skripta.
  2. BIOKEMIJA (6. izd), Školska knjiga, 2013. (prijevod na hrvatski jezik), Relevantna poglavlja iz udžbenika, L. Stryer, J. Berg i J. Tymoczko,.
  3. BIOCHEMISTRY (7. izd.), W. H. Freeman & Co, New York, 2012., Relevantna poglavlja iz udžbenika, J. M. Berg, J. L. Tymoczko i L. Stryer,.
  4. BIOCHEMISTRY (4. izd.), Wiley, New York, 2011., Relevantna poglavlja iz udžbenika, D. Voet i J. G. Voet,.
  5. LEHNINGER PRINCIPLES OF BIOCHEMISTRY (7. izd.), W. H. Freeman & Co, New York, 2017., Relevantna poglavlja iz udžbenika, D. L. Nelson i M. M. Cox,.
Prerequisit for:
Enrollment :
Enrolled : Biochemistry 1
5. semester
Mandatory course - Regular study - Biology and Chemistry Education
Consultations schedule:
  • For consultation hours, please contact the course lecturers.