The accuracy and the fidelity of transmission of genetic information with special emphasis on translation: the error frequency, structural bases of accuracy, optimising the speed and the accuracy, comparing the accuracy of three polymerases (DNA-polymerase, RNA-polymerase; peptidyl-transferase), synthetic and editing mechanisms of aminoacyl-tRNA synthetases, substrate channeling, the active role of the ribosome in protein biosynthesis, proofreading, consequences of mistranslation, adaptive translation, the accuracy of organellar protein synthesis, incorporation of nonstandard amino acids into proteins
Description of instruction methods: lectures and seminars
Description of course/module requirements: seminars, oral presentations
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- COMPULSORY LITERATURE:
D. L. Nelson and M. M. Cox, Lehninger principles of Biochemistry, 7th ed., Worth Publishers, New York, 2017.
J. M. Berg, J. L. Tymoczko, G. J. Gatto, Jr., L. Stryer Biochemisty, 8th ed., W. H. Freeman and Company, New York, 2015.
D. Voet, J. G. Voet, BIOCHEMISTRY 4th ed., J. Wiley & Sons, New York 2011.
N. Budisa, Engineering the Genetic Code, Wiley - VCH Verlag GmbH & Co. KGaA, Weinheim, 2006
Review papers:
Perona JJ, Gruic-Sovulj I. Synthetic and editing mechanisms of aminoacyl-tRNA synthetases. Top Curr Chem. 2014;344:1-41.
Rodnina MV. Quality control of mRNA decoding on the bacterial ribosome. Adv Protein Chem Struct Biol. 2012;86:95-128.
Pechmann S, Willmund F, Frydman J. The ribosome as a hub for protein quality control. Mol Cell. 2013;49(3):411-21.
Pan T. Adaptive translation as a mechanism of stress response and adaptation. Annu Rev Genet. 2013;47:121-37.
Roy B, Jacobson A. The intimate relationships of mRNA decay and translation. Trends Genet. 2013;29(12):691-9.
Davis L, Chin JW. Designer proteins: applications of genetic code expansion in cell biology. Nat Rev Mol Cell Biol. 2012;13(3):168-82.
- ADDITIONAL LITERATURE:
Recent review papers covering new research and methodologies in the field of translation
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