Crystallization - sample preparation for X-ray diffraction experiments, determination of crystal symmetry. Data collection. Evaluation of X-ray diffraction intensities. Phase problem solution. Fourier synthesis. Interpretation of electron density maps, rough model. Refinement of the rough model and adjustment with measured intensities. Primary, secondary, tertiary structure of the macromolecule. Structural evaluation of the model and recognition of the protein biological function by using databases and structural bioinformatics (Cambridge Structural Database, Protein Data Bank, SwissProt, Phyre, etc.).
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- OBVEZNA LITERATURA:
Protein Crystallography, Methods and Protocols, Eds: A. Wlodawer, Z. Dauter, M. Jaskolski , Humana Press, 2017; DOI: 10.1007/978-1-4939-7000-1
B. RUPP - Biomacromolecular crystallography: principles, practice and applications to structural biology. Abingdon, New York: Garland Science, Taylor & Francis Group, 2010.
Li-Ling Ooi - Principles of X-ray Crystallography, , Oxford university press, 2010
McPHERSON - Introduction to macromolecular crystallography, Wiley-Liss, 2003
A. McPherson: Introduction to Macromolecular Crystallography, Wiley-Liss, Hoboken, New
Jersey 2003
D. Blow - Outline of crystallography for biologist, Oxford university press, 2002
A.M. Lesk: Introduction to protein architecture, Oxford University Press, Oxford 2001.
Practical Protein Crystallography, D.E. McRee: Practical Protein Crystallography, 2nd Edition, Academic Press, 1999.
- DOPUNSKA LITERATURA:
revijalni članci i znanstvene monografije
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