1. komponenta

Lecture type	Total
Lectures	30
Seminar	15

COURSE CONTENT:
Basic concepts of gene manipulation. Restriction enzymes. Methods for joining DNA molecules. PCR - methods and applications. Introduction of recombinant DNA into host cell. Basic properties of vectors for DNA cloning. Vector types: plasmids, bacteriophage lambda, vectors for cloning large DNA fragments. Cloning strategies. Genomic DNA cloning. cDNA cloning. Screening strategies. DNA sequencing. Brief overview of genomics, transcriptomics and proteomics. Bacterial and eukaryotic systems for protein overexpression. Protein purification methods. Basics of protein engineering. In vitro mutagenesis (site-specific and random). Directed evolution. Phage display. Fluorescent proteins. In vitro transcription and translation. Transgenic plants and animals. Applications of recombinant DNA technology. Ethical issues in gene manipulation.
Seminars will comprise solving of the problem questions and discussions on examples from scientific and laboratory practice. Students will give oral presentations on the latest accomplishments in selected topics of genetic and protein engineering.

LEARNING OUTCOMES:
1. to explain and recommend procedures for gene cloning
2. to explain and recommend procedures for overexpression and purification of recombinanat proteins
3. to specify and explain methods of protein engineering for obtaining altered and/or improved protein variants
4. to explain and compare research in genomics, transcriptomics and proteomics
5. to explain and recommend methods for detection of protein interactions
6. to specify and explain gene manipulation techniques in bacteria and eukaryotic organisms including creation of transgenic organisms
7. to specify and explain the application of genetic and protein engineering in biotechnology and medicine
8. to present an original scientific paper in the form of oral presentation

1. Lehninger Principles of Biochemistry, D.L. Nelson, M.M. Cox, W. H. Freeman & Co., 2021.
2. Principles of Gene Manipulation and Genomics, S.B. Primrose, R.M. Twyman., Blackwell Publishing, 2006.
3. Odabrani znanstveni radovi s najnovijim dostignućima u području genetičkog i proteinskog inženjerstva, razni autori.
4. Molecular Cloning: a laboratoy manual, J. Sambrook, D.W. Russell., Cold Spring Harbor Laboratory Press, 2001.
5. Directed Molecular Evolution of Proteins, S. Brakmann, K. Johnsson., Wiley-VCH, 2002.

Izborni predmeti izvan odabranih grana - Regular study - Analytical and inorganic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical and physical chemistry
Mandatory course - Regular study - Analytical chemistry and biochemistry
Mandatory course - Regular study - Biochemistry and organic chemistry
Mandatory course - Regular study - Biochemistry and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and physical chemistry
Mandatory course - Regular study - Inorganic chemistry and biochemistry
Izborni predmeti izvan odabranih grana - Regular study - Physical and organic chemistry

Izborni predmeti izvan odabranih grana - Regular study - Analytical and inorganic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Physical and organic chemistry