1. komponenta

Lecture type	Total
Laboratory exercises	60

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE CONTENT: Potentiometric titration of amino acids. Determination of Alcohol-dehydrogenase kinetic parameters for ethanol. Specificity of Alcohol-dehydrogenase towards different alcohols. Inhibition of Alcohol-dehydrogenase activity. Size-exclusion chromatography of biological macromolecules. Size-exclusion chromatography of hemoglobin. Electrophoresis of hemoglobins on the agar gel. Polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE). Electrophoresis of DNA on the agarose gel. Preparation of plasmid DNA from transformed bacterial cells.

LEARNING OUTCOMES:
1. Summarize and explain the basic safety rules for the work in biochemical laboratory.
2. Carry out experiments by themselves using the standard biochemical methods.
3. Operate the standard/basic equipment used in biochemical laboratory.
4. Correlate the characteristics of proteins and nucleic acids with the methods that employ these features for the analysis of macromolecules.
5. Be able to complete data analysis and results interpretations for the biochemical reactions of interest.
6. Apply numerical methods for the extraction of biochemical parameters related to enzyme catalysis.
7. Demonstrate the knowledge and understanding of the biological macromolecules handling procedures aimed to prevent loss of their biological activity.

1. Interna skripta za Praktikum iz biokemije, Zavod za biokemiju, 2007, 13. radno izdanje
   Relevantna poglavlja iz udžbenika J.M. Berg, J.L. Tymoczko, L. Stryer, Biochemistry, 7. izd. W.H. Freeman and Co., New York, 2013.
   ili
   Relevantna poglavlja iz udžbenika L. Stryer, J. Berg, J. Tymoczko, Biokemija, Školska knjiga, 2013. (prijevod 6 izd. na hrvatski jezik)
2. D. Voet, J. G. Voet: Biochemistry, 4. izd., Wiley, New York, 2011.

Mandatory course - Regular study - Chemistry